TRYPAN BLUE

Safety Data Sheet
Emergency Telephone Number: CHEMTREC 1-800-424-9300       For Information call: DUDLEY 732-886-3100       
      DUDLEY CORPORATION
           	     125 Kenyon Drive
       				         	     Lakewood, New Jersey 08701
Effective Date: January 1, 2025	
1. PRODUCT: Syn:  Direct blue 14 CAS No.: 72-57-1   E-C No.:200-786-7 Weight:  960.81g/mol Formula: C34H24N6Na4O14S4

2. HAZARDS: GHS Class:  Carcinogenicity (Category 1B)
 Pictogram: 							
Signal Word:         DANGER
HAZARD STATEMENT: H350: May cause cancer.
PRECAUTIONARY STATEMENT: P201: obtain special instructions before use P202: Do not handle until all safety precautions have been read and understood.P405: Store locked up. P281: Use PPE as required	P308 +P313: If exposed or concerned: contact doctor/physician. P501: Dispose of contents/container to an approved waste disposal plant.

HMIS Class: Health hazard  0, Flammability   0,  Physical hazards   0			
NFPA Rating: Health hazard  0, Fire     0, Reactivity hazard   0

Potential Effects: Eye: Risk of serious damage to eyes.	Skin: Harmful in contact with skin. 
Ingestion: Harmful if swallowed. 		Inhalation: Harmful by inhalation. 
2.3: Other Hazards: none

3. COMPOSITION / INFO: 
Chemical 							 CAS No.	 	Percent		
Tetrasodium 3,3’-[(3,3’-dimethyl[1,1’-biphenyl]-4,4’-diyl)bis(azo)]
bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate]	             		   72-57-1               100%                  

4. FIRST AID: Eyes: rinse with plenty of water as precaution. Skin: Rinse with plenty of soap & water 
Ingestion: Do not induce vomiting without medical advice. Rinse with water. Consult physician.
Inhalation: Move to fresh air.  Consult a physician. Physician: Treat symptomatically and supportively. 

5. FIRE FIGHTING: Info: wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH & full PPE. Irritating & toxic gases may be generated by thermal decomp/combustion. Hazards: Carbon, sulphur, sodium & nitrogen oxides. Extinguishing: Use water spray, dry chem, carbon dioxide, or alcohol-resistant foam. 

6. ACCIDENTAL RELEASE: Info: Use proper PPE as indicated in Section 8. Spills/Leaks: Avoid runoff into storm sewers and ditches which lead to waterways. Clean up spills immediately, observing precautions in the Protective Equipment section. Sweep up, then place into a suitable container for disposal. Avoid generating dusty conditions. Provide ventilation.  Do not let product enter drains. Prevent further leakage or spill is safe to do so.

7. HANDLING & STORAGE: Handling: Wash thoroughly after handling. Wash hands before eating. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Keep container closed when not in use. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.  Storage: Keep container closed when not in use. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances. 

8. EXPOSURE: Use process enclosure, local exhaust ventilation, or engineering controls for airborne levels. 
Chemical 	                           		ACGIH		NIOSH		OSHA-Final PEL’S
Trypan Blue			      	None listed	None listed	None listed. 

PPE: Respiratory: Where risk assessment shows air-purifying respirators are appropriate use a full-face particle respirator type N99 (US) or type P2 (EN 143) respirator cartridges as a backup to engineering controls. If the respirator is the sole means of protection, use a full-face supplied air respirator. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN Hand protection: The selected protective gloves have to satisfy the specifications of EU Directive 89/686/EEC and the standard EN 374 derived from it. Handle with gloves. 0.11mm thick nitrile. Eye protection: Safety glasses  Skin & body: Choose body protection according to the amount and concentration of the dangerous substance at the work place. Hygiene: Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the product.

9. PHYSICAL & CHEM: Appearance: Dark blue to black odorless powder Melting Pt: >300C (>572˚F)  

10. STABILITY & REACTIVITY:  Stability: Stable under normal temperatures and pressures. Avoid: dust generation, excess heat, strong oxidants. Incompatibilities: Oxidizing agents.  Haz Decomp: see section 5

11. TOXICOLOGICAL INFO: RTECS#: QJ6475000.  LD50/LC50: CAS# 72-57-1: Oral, rat: LD50 = 6.200 mg/kg; 

Carcinogenicity:  Not listed by ACGIH, NIOSH, NTP, or OSHA.  Other Studies: unscheduled DNA synthesis
IARC Group 2B- not classified carcinogenicity to humans. (mercury di(acetate))

12. ECOLOGICAL INFO: Toxicity:  LC50-Oryzias latipes >1,000mg/l-48h

13. DISPOSAL CONSIDERATIONS: Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification. Contact a licensed professional waste disposal service to dispose of this material. 
RCRA P-Series: None listed. 	RCRA U-Series: CAS# 72-57-1: Waste number U236. 

14. TRANSPORT REGULATIONS:   RQ = 10 pounds (4.54 kg)
DOT:	UN 3077, Environmentally hazardous substances, solid, n.o.s., (Tetrasodium 3,3’-[(3,3’-dimethyl[1,1’-biphenyl]-4,4’-diyl)bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate]), 9, III, ERG 171
IMDG: Not Regulated	IATA: Not Regulated

15. REGULATORY INFO: SARA 311/312: Chronic Health Hazard
Section 302 (RQ) CAS# 72-57-1: final RQ = 10 pounds (4.54 kg) 
Section 302 (TPQ) None of the chemicals in this product have a TPQ. 
Section 313 This material contains CAS# 72-57-1: Tetrasodium 3,3’-[(3,3’-dimethyl[1,1’-biphenyl]-4,4’-diyl)bis(azo)]
bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate] Rev. 7/1/07which is subject to the reporting requirements of Section 313 of SARA Title III and 40 CFR Part 373.
STATE CAS# 72-57-1 is not present on state lists from CA, MN, FL. 
Mass/PA/NJ Right to Know: CAS# 72-57-1: Tetrasodium 3,3’-[(3,3’-dimethyl[1,1’-biphenyl]-4,4’-diyl)bis(azo)]
bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate] Rev. 7/1/07
California Prop.65: WARNING: This product contains a chemical known to the State of CA to cause birth
defects or other reproductive harm CAS# 72-57-1: Tetrasodium 3,3’-[(3,3’-dimethyl[1,1’-biphenyl]-4,4’-diyl)bis(azo)]
bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate] Rev. 7/1/07
 
EUROPEAN/INTERNATIONAL REGULATIONSEuropean Labeling in Accordance with EC Directives
Hazard Symbols: XN 	WGK (Water Danger/Protection) CAS# 72-57-1 : 2. 
Canada - DSL/NDSL CAS# 72-57-1 is listed on Canada’s DSL List. 
Canada – WHMIS This product has a WHMIS classification of D2A. 
Canadian Ingredient Disclosure List  CAS# 72-57-1 is not listed on the Canadian Ingredient Disclosure List. 

16. DISCLAIMER INFO: DUDLEY CORPORATION provides the information herein in good faith but makes no representation as to its comprehensiveness or accuracy. This document is intended only as a guide to the appropriate precautionary handling of the material by a properly trained person using this.  Individuals receiving the information must exercise their independent judgment in determining its appropriateness for a particular purpose. DUDLEY CORPORATION makes no representations or warranties, either expressed or implied, including without limitation and warranties of merchantability, fitness for a particular purpose with respect to the information set forth herein or to the product to which the information refers. Accordingly, DUDLEY CORPORATION will not be responsible for damages resulting from the use of or reliance upon this information.