4',5'-DIBROMOFLUORESCEIN
Safety Data Sheet
  Emergency Telephone Number: CHEMTREC 1-800-424-9300      For Information call: DUDLEY  732-886-3100
DUDLEY CORPORATION
125 Kenyon Drive				
Lakewood, New Jersey  08701
Effective Date: January 1, 2025
1. PRODUCT ID:  Syn: C.I. 45370.1, Solvent Red 72, 4',5'-Dibromo-3',6'-dihydroxyspiro[isobenzofuran-1(3H), 9'[9 H] xanthen]-3-one; Acid Orange 11; Fluorescein, 4'5'-dibromo-CAS No: 596-03-2.   Weight: 490.11 Formula: C20H10Br2O5

2. HAZARDS ID: GHS Classification: Acute toxicity, Oral (Category 3)	
Pictogram: 				          		
Signal Word:         DANGER
HAZARD STATEMENT(S): H301: Toxic if swallowed.	
PRECAUTIONARY STATEMENT(S) : P264: Wash skin thoroughly after handling.  P330: Rinse Mouth.
P270: Do not drink, eat, smoke, when using this product.	
P301 + P310: If swallowed: Immediately Call Poison Center or doctor/physician
P321: Specific treatment (see supplemental first aid instructions)
P501: Dispose of contents/container to an approved waste disposal plant.

HMIS Classification: Health hazard  2, Flammability   0,  Physical hazards   0			
NFPA Rating: Health hazard  2, Fire     0, Reactivity hazard   0
2.3: Other Hazards: none

3.COMPOSITION/ INFO ON INGREDIENTS: 
Chemical Name         			CAS No. 		Percent		Hazardous
4',5'-Dibromofluorescein     			596-03-2             	100%                              No

4. FIRST AID: Eyes:  Flush eyes with plenty of water as a precaution.   Skin:  flush skin with plenty of soap & water.  Ingestion: If victim is conscious and alert, give water to rinse mouth. Do NOT induce vomiting. NEVER give anything by mouth to an unconscious person. Inhalation: Remove to fresh air immediately. If not breathing, give artificial respiration. Physician: Treat symptomatically & supportively.

5. FIREFIGHTING: Info: Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH & full PPE. irritating & highly toxic gases may be generated by thermal decomp/combustion. Hazards: Carbon oxides, hydrogen bromide. Extinguishing: Use water spray, dry chemical, carbon dioxide, or alcohol-resistant foam. Flash Pt: N/A Auto-ignition Temp: N/A Explosion, Limits: N/A

6. ACCIDENTAL RELEASE: Info: Use proper PPE as indicated in Section 8. Spill/Leaks: Clean up spills immediately, observing precautions in the PPE Section. Sweep up or absorb material, then place into a suitable clean, dry, closed container for disposal.  Avoid generating dusty conditions. Provide ventilation.

7.  HANDLING & STORAGE: Handling: Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation. 
Storage: Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.

8. EXPOSURE CONTROLS, PPE: Controls: Facilities utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate ventilation to keep airborne concentrations low.

CHEMICAL NAME	
ACGIH	
NIOSH	
OSHA – FINAL/Vacated PELs	

4,’5'-Dibromofluorescein	
none listed	
none listed	
none listed	

PPE: Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA’s eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Skin: Wear appropriate protective gloves to prevent skin exposure. Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: A respiratory protection program that meets OSHA’s 29 CFR 1910.134 and ANSI Z88.2 requirements or European Standard EN149 must be followed whenever workplace conditions warrant a respirator use.

9.  PHYSICAL & CHEM: Appearance: Orange powder Boiling Point: 760.00mm Hg Melting Pt: 270-273°C 
Solubility: Slightly soluble 

10. STABILITY & REACTIVITY:  Stability: Stable under normal temp/pressures. Avoid: Incompatibles, dust generation, strong oxidants.  Incompatibilities: Oxidizing agents Hazardous Decomp Products: see section 5

11. TOXICOLOGICAL INFO: RTECS#: LM5200000 LD50: Oral rat <198 mg/kg
Carcinogenicity:  Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA.
Reproductive Effects: Maternal effects: ovaries/fallopian tubes by orl-rat TDLo: 10,500 mg/kg (female 21D pre). 
Mutagenicity: Mutation in microorganisms-Salmonells typhimurium = 100ng/plate 

12. ECOLOGICAL INFO: No information available. 

13.  DISPOSAL CONSIDERATIONS: Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. U.S. EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification. .RCRA P-Series: None listed. RCRA U-Series: None listed. 

14.  TRANSPORT INFO: 
DOT: UN2811, Toxic solids, organic, n.o.s. (4’5’-Dibromo-3’, 6’-dihydroxyspiro[isobenzofuran-1(3H), 9’-[9H]xanthene]-3-one), 6.1, III
IMDG: UN2811, TOXIC SOLID, organic, n.o.s. (4’5’-Dibromo-3’, 6’-dihydroxyspiro[isobenzofuran-1(3H), 9’-[9H]xanthene]-3-one), 6.1, III
IATA: UN2811, Toxic solid, organic, n.o.s. (4’5’-Dibromo-3’, 6’-dihydroxyspiro[isobenzofuran-1(3H), 9’-[9H]xanthene]-3-one), 6.1, III

15.  REGULATORY INFO: SARA 311/312: Acute Health Hazard
Section 302 (RQ & TPQ)None of the chemicals in this material have an RQ or TPQ.
Section 313No chemicals are reportable under Section 313.
STATE CAS# 596-03-2 is not present on state lists from CA, MN, MA, FL. 
PA Right to Know: 4’,5’-Dibromo-3’,6’-dihydroxyspiro[isobenzofuran-1(3H),9’-[9H]xanthene]-3-one  CAS#596-03-2
NJ Right to Know: 4’,5’-Dibromo-3’,6’-dihydroxyspiro[isobenzofuran-1(3H),9’-[9H]xanthene]-3-one  CAS#596-03-2
California Prop.65 components: None of the chemicals are known to cause cancer, birth defects, or any other reproductive harm.

EUROPEAN/INTERNATIONAL REGULATIONS European Labeling in Accordance with EC Directives 
WGK (Water Danger/Protection) CAS# 596-03-2: No information available. 
Canada - DSL.NDSL CAS# 596-03-2 is listed on Canada’s DSL List. 
Canada - WHMIS This product has a WHMIS classification of D2B. 

16. DISCLAIMER INFO: DUDLEY CORPORATION provides the information herein in good faith but makes no representation as to its comprehensiveness or accuracy. This document is intended only as a guide to the appropriate precautionary handling of the material by a properly trained person using this.  Individuals receiving the information must exercise their independent judgment in determining its appropriateness for a particular purpose. DUDLEY CORPORATION makes no representations or warranties, either expressed or implied, including without limitation and warranties of merchantability, fitness for a particular purpose with respect to the information set forth herein or to the product to which the information refers. Accordingly, DUDLEY CORPORATION will not be responsible for damages resulting from the use of or reliance upon this information.